Editorial Book

Book Title: Advances in Plant Biotechnology (Volume 2)

PAID ACCESS | Published on : 15-Oct-2026 | Pages: 40-45 | Doi : https://doi.org/10.37446/vol2book032026/40-45

The Advantage Micro Propagation Leaf Explants Plants of Polygala arvensis (L) -Anxiolytic Effects & Adaptogenic Qualities

• Dr. Mandaloju Venkateshwarlu
• M.Sc., Ph.D. (Botany), M.Sc. (Biotechnology), FSAB, FHAS, SFS, CFE, LL.B., Department of Botany, CUASC, Kakatiya University, Telangana, India.


• Ramesh Kandagatla
• Cosmos, Green, Voltoor, Farm, wanaparthy, TS, India.

Abstract

Tissue culture techniques are now becoming popular as alternative means of vegetative propagation.  Micropropagation involves multiplication of genetically identical individual by asexual reproduction within a short span of time with tremendous potential for the production of high-quality plant-based medicines (Murch et al., 2000). The plant is an annual herb, 10–30 cm tall, with narrow lanceolate leaves arranged alternately. Stems are slender, often branching, and exude a slimy mucilage when crushed. Flowers are small, pale yellow or lilac, clustered in short spikes.  In vitro axillary bud segments and shoot induction was achieved in one of the important medicinal plants. Shoot induction was monitored after 4-6 weeks of inoculation by counting the number of shoots induced from each explant. The advantage with micro propagation is most of the in vitro propagated plants of many important medicinal species were found to be uniform, showing less variation in the secondary metabolic content than their wild counter parts (Annaj and Andrzej S 2005) Shoot induction form axillary bud segments were surface sterilized and inoculated into MS media supplemented with various combinations of BAP L-Glutamil acid and KN. The effect of benzyl amino purine in inducing shoot induction was already reported in some of the important medicinal plants (Komalavalli and Rao, 2000In Vitro propogation of Damaskrose Tabesh et al 2013 & Gago J 2014).  The Axillary bud explants were inoculated on MS basal medium supplemented with various cytokinins i.e., BAP and NAA. Coconut water also had a role in triggering the formation of multiple shoots.  Addition of BAP at 3.0 mg/l concentration or NAA at 3.0 mg/l to the MS basal medium, induced regeneration from the Tendril explants.  Multiple shoot induction was achieved in one of the impartant medicinal plant of Polygalaceae family, Polygala arvensis (L)MS medium supplemented with 1.0 mg/l BAP + 2.0 mg/l NAA and 2.0 mg/l L-Glutamicacid was found to be optimum to induce shoots.  The present study established reliable and reproducible protocol for rapid multiple shoot induction from axilary bud explants of Polygala arvensis (L) using different concentrations and combinations of cytokinins. 

Keywords

Micro Propagation, leaf explants, BAP, L-Glutamic acid, NAA

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