Editorial Book

Book Title: Advances in Plant Biotechnology (Volume 2)

PAID ACCESS | Published on : 04-Aug-2026 | Pages: 6-26 | Doi : 10.37446/vol2book032026/6-26

Recent Trends in Plant Tissue Culture - Transformative Solutions for Crop Improvement

• Nishi Mishra
• Research Associate, Biotechnology Centre, Jawaharlal Nehru Krishi Vishwa Vidyalaya (JNKVV), Jabalpur, Madhya Pradesh, India.


• Manoj Kumar Tripathi
• Biotechnology Centre, Rajmata Vijayaraje Scindia Agricultural University (RVSKVV), Gwalior, Madhya Pradesh, India.


• Radheshyam Sharma
• Biotechnology Centre, Jawaharlal Nehru Krishi Vishwa Vidyalaya (JNKVV), Jabalpur, Madhya Pradesh, India.


• Monika Raghuwanshi
• Department of Agronomy, Jawaharlal Nehru Krishi Vishwa Vidyalaya (JNKVV), Jabalpur, Madhya Pradesh, India.


• Shashank Bhargava
• Biotechnology Centre, Jawaharlal Nehru Krishi Vishwa Vidyalaya (JNKVV), Jabalpur, Madhya Pradesh, India.


• Sonu Sharma
• Department of Plant Pathology, Jawaharlal Nehru Krishi Vishwa Vidyalaya (JNKVV), Jabalpur, Madhya Pradesh, India.

Abstract

Keywords

Plant tissue culture, Artificial intelligence, Bioreactor, Genetic engineering & Nanotechnology

References

Ahuja, A., Tripathi, M. K., Tiwari, S., Tripathi, N., & Tiwari, G. (2021). Recent advancements on callus and cell suspension cultures: An effectual reserve for the production of pharmaceutically significant metabolites. Current Aspects in Pharmaceutical Research and Development, 6(9), 96–111. https://doi.org/10.9734/bpi/caprd/v6/2260C

Altpeter, F., Springer, N. M., Bartley, L. E., Blechl, A. E., Brutnell, T. P., Citovsky, V., Conrad, L. J., Gelvin, S. B., Jackson, D. P., Kausch, A. P., Lemaux, P. G., Medford, J. I., Orozco-Cárdenas, M. L., Tricoli, D. M., Van Eck, J., Voytas, D. F., Walbot, V., Wang, K., Zhang, Z. J., & Stewart Jr., C. N. (2016). Advancing crop transformation in the era of genome editing. The Plant Cell, 28(7), 1510–1520. https://doi.org/10.1105/tpc.16.00196

Anzalone, A. V, Randolph, P. B., Davis, J. R., Sousa, A. A., Koblan, L. W., Levy, J. M., Chen, P. J., Wilson, C., Newby, G. A., Raguram, A., & Liu, D. R. (2019). Search-and-replace genome editing without double-strand breaks or donor DNA. Nature, 576(7785), 149–157. https://doi.org/10.1038/s41586-019-1711-4

Araújo, S. O., Peres, R. S., Ramalho, J. C., Lidon, F., & Barata, J. (2023). Machine learning applications in agriculture: Current trends, challenges, and future perspectives. Agronomy, 13(12), 2976. https://doi.org/10.3390/agronomy13122976

Baulcombe, D. (2004). RNA silencing in plants. Nature, 431(7006), 356–363. https://doi.org/10.1038/nature02874

Bello-Bello, J. J., Mancilla-Álvarez, E., & Spinoso-Castillo, J. L. (2025). Scaling-up procedures and factors for mass micropropagation using temporary immersion systems. In Vitro Cellular & Developmental Biology-Plant, 61(2), 321–332. https://doi.org/10.5897/AJB12.1693

Bhojwani, S. S., & Dantu, P. K. (2013). Tissue and cell culture. In Plant tissue culture: an introductory text (pp. 39–50). Springer.

Bhojwani, S. S., & Razdan, M. K. (1996). Plant tissue culture: Theory and practice, a revised edition. Elsevier Science.

Collard, B. C. Y., & Mackill, D. J. (2008). Marker-assisted selection: An approach for precision plant breeding in the twenty-first century. Philosophical Transactions of the Royal Society B: Biological Sciences, 363(1491), 557–572. https://doi.org/10.1098/rstb.2007.2170

Davey, M. R., Anthony, P., Power, J. B., & Lowe, K. C. (2005). Plant protoplasts: Status and biotechnological perspectives. Biotechnology Advances, 23(2), 131–171. https://doi.org/10.1016/j.biotechadv.2004.09.008

Dodeman, V. L., Ducreux, G., & Kreis, M. (1997). Zygotic embryogenesis versus somatic embryogenesis. Journal of Experimental Botany, 48(8), 1493–1509. https://doi.org/10.1093/jxb/48.8.1493

Eeckhaut, T., Lakshmanan, P. S., Deryckere, D., Van Bockstaele, E., & Van Huylenbroeck, J. (2013). Progress in plant protoplast research. Planta, 238(6), 991–1003. https://doi.org/10.1007/s00425-013-1936-7

Engelmann, F. (2011). Use of biotechnologies for the conservation of plant biodiversity. In Vitro Cellular & Developmental Biology - Plant, 47(1), 5–16. https://doi.org/10.1007/s11627-010-9327-2

Flores, A. I., Morales-Cedeño, L. R., Loeza-Lara, P. D., Schoebitz, M., Orozco-Mosqueda, M. del C., &

Santoyo, G. (2026). Engineering plant-associated microorganisms for bioremediation and sustainable agriculture. Microorganisms, 14(6), 1203. https://doi.org/10.3390/microorganisms14061203

Fowler, M. W. (1982). The large scale cultivation of plant cells. Progress in Industrial Microbiology, 17, 209–220.

Fraley, R. T., Rogers, S. G., Horsch, R. B., Sanders, P. R., Flick, J. S., Adams, S. P., Bittner, M. L., Brand, L. A., Fink, C. L., Fry, J. S., Galluppi, G. R., Goldberg, S. B., Hoffmann, N. L., & Woo, S. C. (1983). Expression of bacterial genes in plant cells. Proceedings of the National Academy of Sciences, 80(15), 4803–4807. https://doi.org/10.1073/pnas.80.15.4803

Gelvin, S. B. (2017). Integration of Agrobacterium T-DNA into the plant genome. Annual Review of Genetics, 51, 195–217. https://doi.org/10.1146/annurev-genet-120215-035320 

George, E. F., Hall, M. A., & De Klerk, G. J. (2008). The anatomy and morphology of tissue cultured plants. In E. F. George, M. A. Hall, & G. J. De Klerk (Eds.), Plant propagation by tissue culture (pp. 465–495). Springer. https://doi.org/10.1007/978-1-4020-5005-3_13

Ghorbanpour, M., & Hadian, J. (2015). Multi-walled carbon nanotubes stimulate callus induction, secondary metabolites biosynthesis and antioxidant capacity in medicinal plant Satureja khuzestanica grown in vitro. Carbon, 94, 749–759. https://doi.org/10.1016/j.carbon.2015.07.056

Haberlandt, G. (1902a). Culturversuche mit isolierten Pflanzenzellen. Sitzungsberichte Der Kaiserlichen Akademie Der Wissenschaften Wien, Mathematisch-Naturwissenschaftliche Klasse, 111, 69–92.

Haberlandt, G. (1902b). Culturversuche mit isolierten pflanzenzellen, sitz-ungsb. Akad. D. Wissensch. Mathermatusch-Naturwissenschaftlicher C169.

Hayat, K., Ali, S., Ullah, S., Fu, Y., & Hussain, M. (2021). Green synthesized silver and copper nanoparticles induced changes in biomass parameters, secondary metabolites production, and antioxidant activity in callus cultures of Artemisia absinthium L. Green Processing and Synthesis, 10(1), 61–72. https://doi.org/10.1515/gps-2021-0010

Hazrati, R., Zare, N., Asghari-Zakaria, R., Sheikhzadeh, P., Shekhawat, M. S., & Singh, R. K. (2025). Enhancing taxane production in Corylus avellana L. cell cultures through elicitation using molybdenum oxide and iron oxide nanoparticles. Industrial Crops and Products, 238, 122269. https://doi.org/10.1016/j.indcrop.2025.122269

Helliwell, C. A., & Waterhouse, P. M. (2005). Constructs and methods for hairpin RNA-mediated gene silencing in plants. In Methods in Enzymology (Vol. 392, pp. 24–35). https://doi.org/10.1016/S0076-6879(04)92002-2

Hesami, M., & Jones, A. M. P. (2020). Application of artificial intelligence models and optimisation algorithms in plant cell and tissue culture. Applied Microbiology and Biotechnology, 104(22), 9449–9485. https://doi.org/10.1007/s00253-020-10888-2

Humbal, A., & Pathak, B. (2023). Harnessing nanoparticle-mediated elicitation in plant tissue culture: A promising approach for secondary metabolite production. Plant Cell, Tissue and Organ Culture, 155(2), 385–402. https://doi.org/10.1007/s11240-023-02612-5

Ikeuchi, M., Ogawa, Y., Iwase, A., & Sugimoto, K. (2016). Plant regeneration: Cellular origins and molecular mechanisms. Development, 143(9), 1442–1451. https://doi.org/10.1242/dev.134668

Jamshidi, S., Yadollahi, A., Arab, M. M., Soltani, M., Eftekhari, M., & Shiri, J. (2020). High throughput mathematical modelling and multi-objective evolutionary algorithms for plant tissue culture media formulation: Case study of pear rootstocks. PLoS ONE, 15(12), e0243940. https://doi.org/10.1371/journal.pone.0243940

Jiménez, V. M. (2005). Involvement of plant hormones and plant growth regulators on in vitro somatic embryogenesis. Plant Growth Regulation, 47(2–3), 91–110. https://doi.org/10.1007/s10725-005-3478-x

Kaur, G., Prakash, P., Srivastava, R., & Verma, P. C. (2020). Enhanced secondary metabolite production in hairy root cultures through biotic and abiotic elicitors. In Plant cell and tissue differentiation and secondary metabolites: Fundamentals and applications (pp. 625–660). Springer. https://doi.org/10.1007/978-3-030-30185-9_38

Khan, A. K., Kousar, S., Tungmunnithum, D., Hano, C., Abbasi, B. H., & Anjum, S. (2021). Nano-elicitation as an effective and emerging strategy for in vitro production of industrially important flavonoids. Applied Sciences, 11(4), 1694. https://doi.org/10.3390/app11041694

Khezri, M., Asghari-Zakaria, R., & Zare, N. (2024). Plant cell and tissue culture: Propagation, improvement,

and conservation of medicinal plants. In Biosynthesis of natural products in plants: Bioengineering in post-genomics era (pp. 267–291). Springer Nature Singapore. https://doi.org/10.1007/978-981-97-2166-5_11

Kim, D. H., Gopal, J., & Sivanesan, I. (2017). Nanomaterials in plant tissue culture: The disclosed and undisclosed. RSC Advances, 7(58), 36492–36505. https://doi.org/10.1039/C7RA07025J

Koti, P., & Bill, T. (2025). Plant tissue culture and genetic transformation in crop improvement. Journal of Bacteriology & Mycology: Open Access, 13(1), 61–69. https://doi.org/10.15406/jbmoa.2025.13.00400

Mishra, N., Tiwari, P. N., Sahu, V. K., & Singh, Y. (2020). Chapter-9 Genome Editing in Plants using CRISPR for Crop Improvement. Recent Trends in Molecular Biology and Biotechnology, 153.

Mishra, N., Tripathi, M. K., Tiwari, S., Tripathi, N., Sapre, S., Ahuja, A., & Tiwari, S. (2021). Cell suspension culture and in vitro screening for drought tolerance in soybean using poly-ethylene glycol. Plants, 10(3), 517. https://doi.org/10.3390/plants10030517

Mridula, M. R., Nair, A. S., & Kumar, K. S. (2018). Genetic programming-based models in plant tissue culture: An addendum to the traditional statistical approach., PLoS Computational Biology 14(2), e1005976. https://doi.org/10.1371/journal.pcbi.1005976

Murashige, T., & Skoog, F. (1962). A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiologia Plantarum, 15(3), 473–497. https://doi.org/10.1111/j.1399-3054.1962.tb08052.x

Murch, S. J., Rupasinghe, H. P. V, Goodenowe, D. B., & Saxena, P. K. (2004). A metabolomic analysis of medicinal diversity in Huang-qin (Scutellaria baicalensis) genotypes: Discovery of novel compounds. Plant Cell Reports, 23(6), 419–425. https://doi.org/10.1007/s00299-004-0862-x

Murthy, H. N., Joseph, K. S., Paek, K.-Y., & Park, S.-Y. (2023). Bioreactor systems for micropropagation of plants: Present scenario and future prospects. Frontiers in Plant Science, 14, 1159588. https://doi.org/10.3389/fpls.2023.1159588

Navya, B. L., & Rajasekharan, P. E. (2025). Pollen cryopreservation in Ashwagandha (Withania somnifera L.). In P. E. Rajasekharan & R. Gowthami (Eds.), Pollen cryopreservation protocols. Humana.

Nazir, S., Jan, H., Zaman, G., Khan, T., Ashraf, H., Meer, B., Zia, M., & Abbasi, B. H. (2021). Copper oxide (CuO) and manganese oxide (MnO) nanoparticles induced biomass accumulation, antioxidants biosynthesis and abiotic elicitation of bioactive compounds in callus cultures of Ocimum basilicum (Thai basil). Artificial Cells, Nanomedicine, and Biotechnology, 49(1), 625–633. https://doi.org/10.1080/21691401.2021.1984935

Niazian, M., & Niedbała, G. (2020). Machine learning for plant breeding and biotechnology. Agriculture, 10(10), 436. https://doi.org/10.3390/agriculture10100436

Paek, K. Y., Chakrabarty, D., & Hahn, E. J. (2005). Application of bioreactor systems for large scale production of horticultural and medicinal plants. Plant Cell, Tissue and Organ Culture, 81(3), 287–300. https://doi.org/10.1007/s11240-004-6648-z

Rashid, R., Khurshid, A., Ferooz, K., Bashir, T., Bhat, K. M., Malik, A. R., Amir, M., Bashir, H., & Bashir, B. (2026). From culture vessels to smart systems: the emergence of intelligent micropropagation technologies. Plant Cell, Tissue and Organ Culture (PCTOC), 165(3), 74.

Rico, S., Garrido, J., Sánchez, C., Ferreiro-Vera, C., Codesido, V., & Vidal, N. (2022). A temporary immersion system to improve Cannabis sativa micropropagation. Frontiers in Plant Science, 13, 895971. https://doi.org/10.3389/fpls.2022.895971

Santra, I., Chakraborty, A., & Ghosh, B. (2024). Evaluation of plumbagin synthesis: Mimicking in vivo plant systems through the application of elicitors inducing stress on in vitro regenerated Plumbago zeylanica L. Plant Cell, Tissue and Organ Culture, 157, 11. https://doi.org/10.1007/s11240-024-02732-6

Sarkar, J., Chakraborty, N., Chatterjee, A., Bhattacharjee, A., Dasgupta, D., & Acharya, K. (2020). Green synthesized copper oxide nanoparticles ameliorate defence and antioxidant enzymes in Lens culinaris. Nanomaterials, 10(2), 312. https://doi.org/10.3390/nano10020312

Sebiani-Calvo, A., Hernández-Soto, A., Hensel, G., & Gatica-Arias, A. (2024). Crop genome editing through tissue-culture-independent transformation methods. Frontiers in Genome Editing, 6, 1490295. https://doi.org/10.3389/fgeed.2024.1490295

Senthil-Kumar, M., & Mysore, K. S. (2011). Caveat of RNAi in plants: The off-target effect. In H. Kodama & A. Komamine (Eds.), RNAi and plant gene function analysis: Methods and protocols (Vol. 744, pp. 13–25). Humana Press. https://doi.org/10.1007/978-1-61779-123-9_2

Shoja, A. A., Çirak, C., Ganjeali, A., & Cheniany, M. (2022). Stimulation of phenolic compounds accumulation and antioxidant activity in in vitro culture of Salvia tebesana Bunge in response to nano-TiO₂ and methyl jasmonate elicitors. Plant Cell, Tissue and Organ Culture, 149(1), 423–440. https://doi.org/10.1007/s11240-022-02251-2

Singh, A. V, Laux, P., Luch, A., Sudrik, C., Wiehr, S., Wild, A. M., & Sitti, M. (2019). Review of emerging concepts in nanotoxicology: Opportunities and challenges for safer nanomaterial design. Toxicology Mechanisms and Methods, 29(5), 378–387. https://doi.org/10.1080/15376516.2019.1566425

Skoog, F. (1957). Chemical regulation of growth and organ formation in plant tissue cultured in vitro. Symp. Soc. Exp. Biol., 11, 118–131.

Steward, F. C., Mapes, M. O., & Mears, K. (1958). Growth and organized development of cultured cells. II. Organization in cultures grown from freely suspended cells. American Journal of Botany, 45(10), 705–708. https://doi.org/10.1002/j.1537-2197.1958.tb10599.x

Thorpe, T. A. (2007). History of plant tissue culture. Molecular Biotechnology, 37(2), 169–180.

Tisserat, B., Esan, E. B., & Murashige, T. (1979). Somatic embryogenesis in angiosperms. In J. Janick (Ed.), Horticultural reviews (Vol. 1, pp. 1–78). Wiley. https://doi.org/10.1002/9781118060742.ch1

Tripathi, M. K., Bele, D., Tiwari, S., Mishra, N., Tripathi, N., & Tiwari, G. (2022). Plantlet regeneration from cultured nodal segments in sandalwood (Santalum album Linn.). In Research Developments in Science and Technology (Vol. 2, pp. 1–21). Book Publisher International.

Tripathi, M. K., Tiwari, G., Tiwari, S., Tripathi, N., Mishra, N., Tomar, Y. S., Uikey, D. S., & Tiwari, S. (2022). Plant Regeneration in Rauvolfia serpentina (L.) Benth via Organogenic Mode. Research Aspects in Biological Science Vol. 7, 7, 124–155.

Tripathi, M. K., Tripathi, N., Tiwari, S., Tiwari, G., Mishra, N., Bele, D., Patel, R. P., Sapre, S., & Tiwari, S. (2021). Optimization of different factors for initiation of somatic embryogenesis in suspension cultures in sandalwood (Santalum album L.). Horticulturae, 7(5), 118. https://doi.org/10.3390/horticulturae7050118

White, P. R. (1943). A handbook of plant tissue culture. Jaques Cattell Press.

Xu, Y., & Crouch, J. H. (2008). Marker-assisted selection in plant breeding: From publications to practice. Crop Science, 48(2), 391–407. https://doi.org/10.2135/cropsci2007.04.0191

Zhang, F. (2019). Development of CRISPR-Cas systems for genome editing and beyond. Quarterly Reviews of Biophysics, 52, e6.

Zhang, Q., Deng, D., Dai, W., Li, J., & Jin, X. (2020). Optimisation of culture conditions for differentiation of melon based on artificial neural network and genetic algorithm. Scientific Reports, 10(1), 3524. https://doi.org/10.1038/s41598-020-60278-x

Zhang, Z.-Y., & Zhong, J.-J. (2004). Scale-up of centrifugal impeller bioreactor for hyperproduction of ginseng saponin and polysaccharide by high-density cultivation of Panax notoginseng cells. Biotechnology Progress, 20(4), 1076–1081. https://doi.org/10.1021/bp0499451

Zhu, H., Li, C., & Gao, C. (2020). Applications of CRISPR–Cas in agriculture and plant biotechnology. Nature Reviews Molecular Cell Biology, 21(11), 661–677. https://doi.org/10.1038/s41580-020-00288-9.