OPEN ACCESS | Published on : 23-Mar-2026 | Pages: 94-110 | Doi : 10.37446/volbook022025/94-110
Livestock Infectious diseases in animals impose substantial economic and public health burdens on livestock, poultry, and aquaculture systems. Conventional diagnostic methods, including PCR and immunoassays, are limited by high cost, operational complexity, and long turnaround times. The CRISPR-Cas system provides a rapid sensitive detection platform for diagnostics utilizing the collateral cleavage activity. This chapter examines CRISPR diagnostics platforms, their mechanisms, and integration with amplification-free assays, and the point of care application which includes, Cas12a fluorometric/LFA detection of canine tick-borne pathogens (Ehrlichia, Babesia with 100% sensitivity and specificity), Cas14a paper-based ASFV biosensors (LOD 5copies/µL), Multiplex RT-RPA-Cas13a for AIV subtypes (LOD 1-10 copies/µL), RPA-Cas12a for Brucella (LOD 1copy/µL), and RPA-Cas12a for lumpy skin disease virus with 96% sensitivity. These technologies are particularly suited for deployment in resource-limited settings, enabling rapid on-site surveillance and disease control, these tools enable on-farm surveillance, variant differentiation, preventive measures and food security.
CRISPR, Veterinary science, Diagnostics, LFA, Fluorescence, Cas system
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